A two unit antisense RNA cassette test system for silencing of target genes

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A two unit antisense RNA cassette test system for silencing of target genes.

This communication describes a two unit antisense RNA cassette system for use in gene silencing. Cassettes consist of a recognition unit and an inhibitory unit which are transcribed into a single RNA that carries sequences of non-contiguous complementarity to the chosen target RNA. The recognition unit is designed as a stem-loop for rapid formation of long- lived binding intermediates with targ...

متن کامل

Silencing genes in the kidney: antisense or RNA interference?

Targeting genes in disease has long been a sought-after holy grail, with the concept of gene therapy promising a magic bullet for single gene mutation or deletion disorders. Replacing deficient or non-functional genes with the active form has proven more difficult to achieve than originally hoped. However, a subset of gene therapy termed ‘gene silencing’ is being developed, which is altogether ...

متن کامل

A copper-controlled RNA interference system for reversible silencing of target genes in Trichoderma reesei

Background Trichoderma reesei is a primary lignocellulosic enzyme producer in industry. However, the mechanisms underlying cellulase synthesis as well as other physiological processes are insufficiently understood partly due to the sophisticated process for its genetic manipulation. Target gene knockdown by RNA interference (RNAi) is a powerful tool for genetic research and biotechnology in euk...

متن کامل

A genome-wide inducible phenotypic screen identifies antisense RNA constructs silencing Escherichia coli essential genes.

Regulated antisense RNA (asRNA) expression has been employed successfully in Gram-positive bacteria for genome-wide essential gene identification and drug target determination. However, there have been no published reports describing the application of asRNA gene silencing for comprehensive analyses of essential genes in Gram-negative bacteria. In this study, we report the first genome-wide ide...

متن کامل

Polymorphisms of Antioxidant’ Genes as a Target for Diabetes Management

Diabetes mellitus (DM) is one of the most important health problems with increasing prevalence worldwide. Oxidative stress that is a result of imbalance between reactive oxygen species (ROS) generation and antioxidant defense mechanisms has been demonstrated as a main pathology in DM. Hyperglycemia-induced ROS productions can induce oxidative stress through four major molecular mechanisms inclu...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1997

ISSN: 1362-4962

DOI: 10.1093/nar/25.16.3218